Basic Laboratory Techniques Safety And Hematocrit

Basic Laboratory Techniques Safety And Hematocrit The hematocrit is normally ordered as a part of the complete blood count. It is important in evaluating anemia and polycythemia, monitoring the recovery from dehydration, the effectiveness of treatment for anemia, the ongoing bleeding to check its severity, etc. In this practical, hematocrit is used to determine if the patient has anemia by means of red cell indices MCV, MCH and MCHC. For red blood cell count, it is used to evaluate any type of decrease or increase in the number of red blood cells as measured per liter of blood. It is a parameter interprets in conjunction with hematocrit. Both decreased hematocrit and decreased RBC count indicate anemia. (D) Results Hematocrit (HCT) Record the red blood cell indices in the table below. Reading SI Unit RBC 8.55 ÃÆ'- 1012 RBC/L Hemoglobin 11.0 g/dL Your HCT value 26 % The red blood cell indices are used to classify anemias. Find out their formulae and calculate the MCV, MCH MCHC. Compare them to the reference values of a normal adult female. Red cell indices Formula Calculation (units) Reference range Mean cell volume (MCV) Hematocrit (%) ÃÆ'- 10 RBC _26 ÃÆ'- 10 8.55 MCV= 30.4 fL 86 98 fL Mean cell hemoglobin (MCH) Hemoglobin(grams) ÃÆ'- 10 RBC __11__ ÃÆ'- 10 8.55 MCH= 12.87 pg 27 32 pg Mean cell hemoglobin concentration (MCHC) Hemoglobin(grams) ÃÆ'- 100 Hematocrit (%) __11__ ÃÆ'- 100 26 MCHC= 42.3 % 32 37 % (*delete as appropriate and suggest whether blood smear A or B provided in the lab would belong to this sample) As your calculated MCV suggest, the RBCs are macrocytic / normocytic / microcytic *. As your calculated MCH suggest, the hemoglobin in the RBCs are within / below / above* the average weight. As your calculated MCHC suggest, the RBCs are normochromic / hypochromic / hyperchromic*. Blood smear A / B* belongs to this patient. Cell count and hemacytometer RBC Counts Averaging no. of RBCs in 2 one square millimeter Square No. of RBC counted 1 178 2 164 Total RBCs counted 342 Averaged RBC counted 171 Calculate the RBC count in the sample: = Average no. RBCs counted in 1 big square ÃÆ'- Dilution factor Area counted (mm2) ÃÆ'- Depth (mm) RBC count = 171 ÃÆ'- 1000 ikikikiiiiiiu 0.2 (mm2) ÃÆ'- 0.1 (mm) RBC count = 8.55 ÃÆ'- 1012 (units= /L ) (E) Quality Assessment Obtain results from the class and do the statistics.(Refer to Basic Clinical Lab Techniques pp. 79 81) ÃŽÂ £X_ n (117+160+174+142+166+161+181+183+166+143+164+159+148+162+225+135+165+120+ 168+128+271+104+171+168+171+182+170+176+181+116+172) à · 31 RBC counts is 162.87 . n Test Value (mg/dL) X Deviation Squared ( x)2 1 117 2104.15 2 160 8.24 3 174 123.86 4 142 435.60 5 166 9.79 6 161 3.50 7 181 328.66 8 183 405.18 9 166 9.79 10 143 394.86 11 164 1.27 12 159 14.98 13 148 221.15 14 162 0.76 15 225 3860.01 16 135 776.79 17 165 4.53 18 120 1837.92 19 168 26.31 20 128 1215.98 21 271 11691.89 22 104 3465.79 23 171 66.08 24 168 26.31 25 171 66.08 26 182 365.92 27 170 50.82 28 176 172.37 29 181 328.66 30 116 2196.89 31 172 83.34 ÃŽÂ £( x)2__ n 1 30297.48__ 30 = 1009.916 à ¢Ã‹â€ Ã… ¡Variance à ¢Ã‹â€ Ã… ¡1009.916 31.78 2s = 63.56 +2s = 226.43 -2s = 99.31 3s = 95.34 +3s = 258.21 -3s = 67.53 10. Construction of a Levey-Jenning s Chart using the mean and standard deviation(s) from above. Indicate the mean value, +/- 1s, +/-2s, +/-3s on the appropriate lines. Using the RBC data from the class, plot the values from all students. Levey-Jennings Chart of RBC counts in class SD of RBC counts (G) Practical Review Questions Label the hemacytometer diagram below with its parts. (p208) Indicate the areas usually used for WBC count with W RBC count with R and Platelet count.p 1. What does the hematocrit measure? The hematocrit is a test determining the patients red cell volume found in whole blood and, thus, the bloods oxygen carrying capacity. It is expressed as a percentage by volume. 2. Give the hematocrit reference values for males, females, and newborns. Males: 42 52 % Females: 36 48 % Newborns: 51 61 % 3. Name a condition that could cause a decreased hematocrit value. Anemias 4. Explain the hematocrit procedure 1. Obtain patients blood and prepare the specimen. Gently mix the capillary blood by inverting the tube until all sediments disappear. Blood sample is drawn into a heparinixed microhematocrit tube by capillary action. Load the microhematocrit tube by holding it end downward to allow gravity to facilitate loading of the tube. The tube should be filled to about 3/4 full. Seal one end of the tube with a small amount of clay material at a 90 ° angle. 2. Centrifuge the samples. Please the sealed microhematocrit tube into the rotor of the microhematocrit centrifuge, with the sealed end against the rubber gasket. Duplicate samples should be placed opposite each other in order to balance the centrifuge. Securely fasten the flat lid on top of the capillary tubes. Spin the tubes for 2 to 4 minutes at 10000 rpm. After the centrifuge has stopped, open the top and remove the cover plate. 3. Reading and reporting the Hematocrit value. Inspect the tube to see if there is any leakage after centrifugation. Place the tube on the hematocrit reader. Place tube in the groove with the top of the seal-ease (bottom of the packed cells) lined up with the bottom line of the reader.   Move the slider bar to the border between the packed red cells and the plasma.   Read the percentage reading beside the slide bar. 5. Blood enters the capillary tube by what action? Blood enters by capillary action. 6. Why must the capillary tube be sealed securely? In the process of spinning, RBCs and a small amount of plasma will be forced from the tube, a false result may be resulted because of incomplete sealing of tube. Therefore, sealing the tube securely can create a pressure inside the tube which prevents the content from forcing out during centrifugation. 7. What is the usual length of time for centrifugation of the hematocrit tubes? The hematocrit tubes are usually centrifugated for 2 to 4 minutes at 10000rpm. 8. What safety precautions should be observed when performing a hematocrit? Standard precaution should be observed. 9. What technical factors can affect the quality of hematocrit results? Blood sample should be well-mixed before microhematocrit tubes are filled. Avoid bubbles when filling blood sample into the microhematocrit tube. Microhematocrit tubes should be filled at least three-fourths full. The microhematocrit tubes should be completely sealed before centrifugation. Read the hematocrit value at the top of the red cell layer, not at the top of buffy coat. 10.Case Study 1 A hemoglobin and hematocrit test performed in a pediatric clinic on a 2-year-old boy gave results of 110 g/L hemoglobin and 0.33 L/L hematocrit. Do these results agree? What is the boys general health status based on the hematology results? From the data provided by the case, the mean cell hemoglobin concentration (MCHC) of the child: __11__ ÃÆ'- 100 33 = 33% The MCHC is within the normal range, showing the child is free from anemia. However, according to World Health Organization, a hemoglobin concentration of less than 110 g/L and a hematocrit value of less than 33% were used as a cut-off for anemia for children aged 0.5 to 5[1]. Therefore, for the above case, although the child does not have anemia at this moment, his marginal hemoglobin and hematocrit values agree and both show that he has a high risk of suffering anemia. Age or gender group Hemoglobin (g/dL) Hematocrit (%) Children (0.5-4) Children (5-12) Children (12-15) Adult Men Non-pregnant Women Pregnant Women

A Comparison of Research Methods

Sociology is the study of human relationships and institutions (UNC, 2013). In order to enrich our understanding of key social processes, sociologists carefully gather and analyze evidence about social life. Most sociological research involves â€Å"field work† that has been designed to most closely represent the characteristics of a population (UNC, 2013). This process involves the utilization of a wide variety of research methods. Some of these methods include conducting surveys of large groups, observing people in social settings, deciphering historical data and analyzing videotaped interactions.For purposes of this paper, we are going to take a more in-depth look at two research methods used in sociology. The first research method we will look at is correlation research. A correlation is a relationship between two variables (Cliffsnotes, 2013). They can be behaviors, events, characteristics or attitudes. Correlation research attempts to determine if a relationship exists b etween two variables and exactly what the degree of that relationship is. A sociologist can use any number of other research methods to determine if a correlation exists.Correlations are either positive (to +1.  0), negative (to -1. 0) or nonexistent (0. 0) (Cliffsnotes, 2013). A positive correlation is one in which the variables either increase or decrease together. A negative correlation on the other hand is one in which the variables go in different directions. If one increases, the other one decreases. If a correlation is nonexistent, no relationship exists between the variables. Correlation data does not indicate a cause and effect relationship (Cliffsnotes, 2013). In other words, one variable does not cause the other; it only indicates that both variables are somehow related to one another.Changes in the value of one variable will reflect changes in the value of the other. The second research method we will analyze is survey research. Survey research usually involves intervi ewing or administering questionnaires to a large number of people. This can be accomplished in person, by mail or over the telephone. Sociologists are able to customize the questions within the survey to make them pertinent to the study at hand. Once all data has been collected, the sociologist will analyze it to pinpoint similarities, differences and trends. They will then make predictions about the population being studied.One advantage to this type of research is that it allows sociologists to collect data from a large group in a relatively short period. This type of research method is also relatively inexpensive. If conducted by mail, the surveys allow the respondents the convenience of completing them on their own time. The mail surveys are also anonymous, which may provide for responses that are more truthful. Some disadvantages to this type of research method are that the responses may not always be reliable, it can be time consuming to decipher the results, and not all respo ndents will reply.A1. Philosophical Justifications for Sociological Methods The survey method is one of the best methods of research for gathering large amounts of information quickly and affordably (Jones, 2013). This method allows for a broader scope of study with greater efficiency (Pearson, 2013). Subjects may remain anonymous, which in turn can produce more truthful responses to questions (Jones, 2013). Due to the fact that the sociologist may not be present during some of the surveys, this method helps to eliminate bias in the interpretation of the results (Jones, 2013).This method of research is seen as highly reliable in that the data collected can be easily coded and analyzed (Pearson, 2013). Survey methods are a valuable adjunct to other research methods. They play an important role in confirming more qualitative research. The survey method can help to identify areas that need further research and reveal broad patterns that exist that could otherwise be overlooked by resea rchers relying solely on qualitative methods (Pearson, 2013). B. Anthropological Research MethodsAnthropology comes from the Greek, it literally means â€Å"the study of the human† (Malinowski, 2013). Cultural anthropology is the study of human cultures, their practices, beliefs and values. Anthropologists accomplish these studies through various methods of research. These methods include (but are not limited to) participant observation, cross-cultural comparison, survey research, interviews and historical analysis. In this paper, we are going to take a closer look at two different forms of research used within the field of cultural anthropology.The first method of anthropological research that we will look at is participant observation. Participant observation is an immersion method of research where the researchers immerse themselves into the culture that they are studying. They will live within this society for the duration of the study (Jones, 2013). By doing this, the re searcher is able to get first-hand experience through observation and participation in ceremonies, rituals, storytelling, language and meals within these cultures (Jones, 2013).They record their findings through voice recordings, photos, videos and journals. The success of participant observation is reliant upon the particular culture’s acceptance of the investigator into their society (Jones, 2013). The second method of research that we will discuss is cross culture comparison. This method searches for comparable culture patterns amongst multiple societies. Testable hypotheses are utilized to try to establish statistical correlations amongst cultures (Gillies and Kinzer, 2009). This methodology was greatly facilitated via the work of George Peter Murdock.Murdock was an American anthropologist known for his comparative studies. He developed the Human Relations Area Files (HRAF), which is an index that contains many of the world’s known societies (Gillies and Kinzer, 20 09). There are two goals within this research method: 1) to describe the distribution and range of cultural variation that exists between the ethnographies recorded, and 2) to test the theories and hypotheses that have been proposed in order to explain the variations amongst cultures (Gillies and Kinzer, 2009).B1. Philosophical Justifications for Anthropological Methods Participant observation allows the researcher to gain an inside look at the inner workings of a culture. By immersing oneself into a culture for an extended period of time, the researcher will gain more knowledge of the society (Jones, 2013). This technique will also produce a greater sense of trust from the members of the culture. This can in turn, provide for information that is more accurate and provide for a more comprehensive ethnography (Jones, 2013).Ethnographies allow people to gain an understanding of a particular culture and the appropriate ways to interact with that culture (Jones, 2013). They also can ser ve as a historical record of cultures that may be endangered or extinct. Some individuals may see the participant observation method as an invasion of privacy. Researchers however, go to great lengths to ensure that the utmost respect is given to all members of these cultures. Permission from the cultural leaders is always sought out prior to initiation of this type of research (Jones, 2013). C. Compare and Contrast ApproachesSociology is the study of the development, structure, interaction and behavior of organized groups of human beings (Diffen, 2012). Anthropology is the study of human beings and their ancestors through time in terms of physical characteristics, culture, environment and social relations (Diffen, 2012). Sociologists study societies, while anthropologists study cultures. Both of these areas of study use secondary analysis as a research method. They each can utilize the same types of material and information. They even use some of the same methods of gathering this information.The difference between them is in how the information is utilized and reported. Traditionally, sociology studies modern, civilized and complex societies (Bhatt, 2012). Anthropology on the other hand, traditionally studies simple, more primitive, non-literate societies (Bhatt, 2013). Sociology studies both large and small societies while anthropology tends to focus more on smaller societies. When it comes to research styles, anthropology stresses immersion in native life while sociology tends to stress distance from the object of study (Dilipchandra, 2012).Sociology treats data quantitatively while anthropology treats data qualitatively. Sociology emphasized that human behavior can be measured and that that measurement is reliable (Dilipchandra, 2012). Anthropology touts that a large part of human behavior is beyond the realm of measurement (Dilipchandra, 2012). Due to changes in our world, sociology and anthropology are converging (McGraw-Hill, 2013). Sociologists can no w be found doing research in developing countries and due to industrialization, anthropologists can be found doing research in industrialized societies (McGraw-Hill, 2013).

The Creatures Of The Mountains Of Ankar - 1220 Words

High up on the mountains of Ankar, resided an ancient breed of dragon. They were fire dragons, and were used to the frosty, high peaks they inhabited. Living for thousands upon thousands of years, these creatures ruled atop their mountain. Ruling as they please, and doing as they liked to the beings below them. They soared high in the sky without a fear in the world. For a long while, however, a dragon egg has not appeared. Until one day, as if a gift from heaven, a dragon egg appeared at the foot of the mountain. The egg was smaller and differently colored than the ones that they were used to. The dragons brought the egg atop the mountain and awaited for the appearance of another of their kin. They kept the egg inside a cave. Every day,†¦show more content†¦It was a feeling of happiness. They watched in awe as the first crack appears. Light emanated from the egg. Finally, the baby poked its head out of its shell. Its eyes were closed as it breached the shell but then they w ere wide open. Its eyes were filled with curiosity as it surveyed its surroundings. It made a small purring sound and yawned. The dragons awed, making a sound of approval. Some ran outside and blew scorches of fire into the air, signifying the appearance of another dragon. Other dragons flying through the air looked towards the commotion and joined the crowd. When everything had quieted down, one dragon began to speak. His scales were a bright red-orange and his eyes were furrowed in concentration, even to the point of seeming like anger. His voice resonated and sounded like he was talking through water. â€Å"Look, his scales aren’t even red.† The other dragons were so excited about the birth of a baby dragon that they didn’t see that what he said was true. The cave began to be filled with concerned murmurs. One of the dragon elders stepped forward, Grandine, and grabbed the baby dragon gently. She cradled him in her arms and spoke to the crowd. â€Å"We cannot judge the child now, we can only wait and see.† The noise quieted down, but there was still murmurs. Decades passed by and the dragon slowly grew. He adapted well into the colony of fire dragons and everyone had called him Criosis but there was still others who saw him as an outsider.

The Placement Of Foreshadowing In F. Scott Fitzgeralds...

In the world of writing, novelists tend to place many literary devices to present the audience reading with a notion regarding a particular person, place, or thing in their novel. As literary devices can be intentionally installed as well as unintentionally, authors tend to leave it up to the audience to decide whether or not it was a choice or just a coincidence. In F. Scott Fitzgeralds The Great Gatsby, many literary devices can be noticed, but the idea of foreshadowing particularly raises attention. Foreshadowing can be defined as a warning or indication of a possible future event. In The Great Gatsby, this literary device can be seen throughout the novel as a hint leading to events that would later occur. Foreshadowing in this novel†¦show more content†¦When thinking of the weather, what usually appears after rain is the beautiful breeze and extravagant sun. The warmth of the sun tends to put people at ease and creates a moment where they can be clear minded. Gatsby was able to overlook his nervous state and remind Daisy of who he was before when they first met. This change in the weather mirrors the loosening of tension between Gatsby and Daisy. Pathetic fallacy also takes place in chapters seven, and eight. During chapter seven or the hottest time of the summer, Gatsby confronts Tom about the love he and Daisy have found again. This being the hottest day of the summer does not seem to be a coincidence. During extremely hot weather, people tend to get stressed and fed up quicker. In this case, the heat intensifies the existing tension between Tom and Gatsby as they fight over Daisy. Foreshadowing can be used to see that Gatsbys and Daisys relationship will soon come to an end. The moment Daisy states that she still loved Tom, Gatsby realizes the mistake he has made in assuming nothing has changed over the time they have been apart.When experiencing intense heat, the best option is to find somewhere cool. Between chapters seven and eight, seven is the heat and eight is the cool down. In chapter eight, after the destructive summer fight in chapter seven, it is now autumn. Autumn is known heavily for the falling of leaves, the cooling of weather, and